Single Nucleotide Polymorphisms: Methods and Protocols

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Single Nucleotide Polymorphisms:...

Single Nucleotide Polymorphisms: Methods and Protocols

Name: Single Nucleotide Polymorphisms: Methods and Protocols
ISBN: 9781592593279

Lisa D. Brooks (auth.), Pui-Yan Kwok MD, PhD (eds.)

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Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.

카테고리:

Medicine

년:

2003

판:

출판사:

Springer New York

언어:

english

페이지:

283

ISBN 10: